| 质粒名称: | TRUPATH Triple Gai1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 196048 |
| 存储实验室: | Justin English |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1(+) |
| 载体出品公司: | Genscript |
| 空载体大小: | 5140bp |
| 完整质粒大小: | 9868bp |
| 载体类型: | 哺乳细胞表达, Luciferase |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称1: | GAlphai1-RLuc8、GNAI1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 2031bp |
| 基因突变: | RLuc8 and flanking SGGGGS linkers have been inserted at amino acid position 91 of the alpha subunit |
| 启动子: | CMV |
| 克隆方法: | 未知 |
| 5’克隆位点: | AgeI(未破坏) |
| 5’测序引物 | gccacgttgtgagttggatag |
| 3’测序引物 | TAGAAGGCACAGTCGAGG |
| 插入基因名称2: | GGamma9-GFP2、GNG9 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | (bp)939 |
| 启动子: | CMV |
| 标签融合： | GFP2 and GSAG linker (N terminal on insert) |
| 克隆方法: | 未知 |
| 5’测序引物 | gagggcagaggaagtcttc |
| 3’测序引物 | GATTTAGGTGACACTATAG |
| 插入基因名称3: | GBeta3、GNB3 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | (bp)1020 |
| 启动子: | CMV |
| 克隆方法: | 未知 |
| 5’测序引物 | CGCAAATGGGCGGTAGGCGTG |
| 3’测序引物 | ccggtagggccgggattc |
|
文献引用方法 |
|
| 材料和方法部分: | TRUPATH Triple Gai1 was a gift from Justin English (Addgene plasmid # 196048 ; http://n2t.net/addgene:196048 ; RRID:Addgene_196048) |
| 参考文献部分: | TRUPATH, an open-source biosensor platform for interrogating the GPCR transducerome. Olsen RHJ, DiBerto JF, English JG, Glaudin AM, Krumm BE, Slocum ST, Che T, Gavin AC, McCorvy JD, Roth BL, Strachan RT. Nat Chem Biol. 2020 May 4. pii: 10.1038/s41589-020-0535-8. doi: 10.1038/s41589-020-0535-8. 10.1038/s41589-020-0535-8 PubMed 32367019 |
买家导航
TRUPATH Triple Gai1载体质粒基本信息
TRUPATH Triple Gai1质粒图谱载体图谱和TRUPATH Triple Gai1载体序列质粒序列多克隆位点信息
TRUPATH Triple Gai1质粒载体简介
TRUPATH Triple Gai1质粒序列载体序列
LOCUS TRUPATH Triple Gai1 10159 bp DNA circular SYN 25-AUG-2024
DEFINITION Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit
(GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components
of a BRET2 biosensor for studying heterotrimeric G proteins.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10159)
AUTHORS Olsen RHJ, DiBerto JF, English JG, Glaudin AM, Krumm BE, Slocum ST,
Che T, Gavin AC, McCorvy JD, Roth BL, Strachan RT
TITLE TRUPATH, an open-source biosensor platform for interrogating the
GPCR transducerome.
JOURNAL Nat Chem Biol. 2020 May 4. pii: 10.1038/s41589-020-0535-8. doi:
10.1038/s41589-020-0535-8.
PUBMED 32367019
REFERENCE 2 (bases 1 to 10159)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem
Biol. 2020 May 4. pii: 10.1038/s41589-020-0535-8. doi:
10.1038/s41589-020-0535-8."
FEATURES Location/Qualifiers
source 1..10159
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1937..1990
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
primer_bind complement(3007..3024)
/label=SP6
/note="SP6 promoter, forward primer"
misc_feature 3036..3609
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
misc_feature 3059..3611
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(3203..3220)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 3430..3449
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
primer_bind complement(5750..5767)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5756..5980
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 6026..6454
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6113..6132)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6323..6344
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6463..6483)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 6468..6797
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 6648..6783
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6710..6729
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 6864..7658
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6918..6937)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 7528..7547
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 7832..7953
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7869..7888)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7923..7942
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(8002..8018)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8002..8018)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8015..8037)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8026..8042
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8050..8080)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8095..8116
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8233..8250)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8404..8992)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8484..8503)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9163..10023)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9786..9805
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10024..10128)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttatgg gagagatgga gcagctgagg caggaggcag agcagctgaa
961 gaagcagatc gccgacgcca gaaaggcctg cgcagatgtg accctggcag agctggtgag
1021 cggactggag gtggtgggaa gggtgcagat gaggaccagg agaacactga ggggccacct
1081 ggccaagatc tacgccatgc actgggccac agactctaag ctgctggtgt ccgcctctca
1141 ggacggcaag ctgatcgtgt gggatagcta taccacaaac aaggtgcacg caatccccct
1201 gcgcagctcc tgggtcatga cctgtgccta cgccccttcc ggaaatttcg tggcatgcgg
1261 aggactggat aacatgtgct ctatctataa tctgaagagc cgggagggca acgtgaaggt
1321 gtctagagag ctgagcgccc acaccggcta cctgtcttgc tgtcggttcc tggacgataa
1381 caatatcgtg acatctagcg gcgacaccac atgcgcactg tgggacatcg agaccggaca
1441 gcagaagaca gtgtttgtgg gccacacagg cgactgtatg tccctggccg tgtctccaga
1501 tttcaatctg tttatctccg gagcatgcga cgcatctgcc aagctgtggg atgtgcggga
1561 gggcacctgt agacagacct tcacaggcca cgagagcgac atcaacgcca tctgcttctt
1621 tcccaatggc gaggccatct gcacaggcag cgacgatgcc tcctgtaggc tgttcgacct
1681 gcgcgccgat caggagctga tctgcttttc tcacgagagc atcatctgtg gcatcacctc
1741 cgtggccttt agcctgtccg gcaggctgct gttcgcagga tatgacgact tcaactgcaa
1801 cgtgtgggac tccatgaagt ctgagagggt gggcatcctg agcggccacg ataatcgcgt
1861 gtcctgtctg ggagtgaccg cagacggaat ggcagtggcc acaggcagct gggattcctt
1921 tctgaagatc tggaacgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa
1981 tcccggccct accggtatgg tgtccaaggg agaggagctg ttcaccggag tggtgcctat
2041 cctggtggag ctggacggcg atgtgaatgg ccacaagttt agcgtgtccg gagagggaga
2101 gggcgacgca acctacggca agctgacact gaagttcatc tgcaccacag gcaagctgcc
2161 cgtgccttgg ccaaccctgg tgaccacact gtcctacggc gtgcagtgtt tttctaggta
2221 tcctgaccac atgaagcagc acgatttctt taagtctgcc atgccagagg gctacgtgca
2281 ggagcggaca atcttcttta aggacgatgg caactataag accagagccg aggtgaagtt
2341 cgagggcgac acactggtga acaggatcga gctgaagggc atcgacttta aggaggatgg
2401 caatatcctg ggccacaagc tggagtacaa ctataatccc cacaacgtgt atatcatggc
2461 cgataagcag aagaacggca tcaaggtcaa tttcaagatc cgccacaata tcgaggacgg
2521 cagcgtgcag ctggccgatc actaccagca gaacacccca atcggcgacg gacccgtgct
2581 gctgcctgat aatcactatc tgtttacaca gtctgccctg agcaaggacc ccaacgagaa
2641 gcgggatcac atggtgctgc tggagttcgt gaccgcagca ggcatcacac tgggcatgga
2701 tgagctgtac aagggcagcg ccggcaccat ggcacaggac ctgtccgaga aggatctgct
2761 gaagatggag gtggagcagc tgaagaagga ggtgaagaat acaagaatcc ccatcagcaa
2821 ggccggcaag gagatcaagg agtatgtgga ggcccaggcc ggcaacgacc ctttcctgaa
2881 gggcatccca gaggataaga atccctttaa ggagaagggc ggctgcatca tcagctgacc
2941 gcggcgtacg aggcctgcat gctccggacc tgcaggttcg aagtcgacag atctcaattg
3001 gggcccctat agtgtcacct aaataattcc gcccccccct ctccctcccc cccccctaac
3061 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
3121 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
3181 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
3241 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
3301 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
3361 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
3421 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
3481 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
3541 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
3601 cgatgataat atgggctgca ccctgtccgc agaggataag gcagcagtgg agcggtctaa
3661 gatgatcgat cggaatctga gagaggacgg cgagaaggcc gccagagagg tgaagctgct
3721 gctgctggga gcaggagagt ctggcaagag caccatcgtg aagcagatga agatcatcca
3781 cgaggccggc tactctgagg aggagtgtaa gcagtacaag gccgtggtgt attctaacac
3841 aatccagagc atcatcgcca tcatcagggc aatgggaaga agcggaggag gaggctccat
3901 ggcatctaag gtgtatgacc ctgagcagag gaagcgcatg atcaccggac cacagtggtg
3961 ggcaaggtgc aagcagatga acgtgctgga ttcctttatc aattactatg actctgagaa
4021 gcacgccgag aacgccgtga tcttcctgca cggcaatgcc acaagctcct acctgtggag
4081 gcacgtggtg ccccacatcg agcctgtggc aaggtgtatc atccctgatc tgatcggcat
4141 gggcaagagc ggcaagtccg gcaatggcag ctataggctg ctggaccact acaagtatct
4201 gaccgcctgg ttcgagctgc tgaacctgcc aaagaagatc atctttgtgg gacacgactg
4261 gggagcagca ctggcattcc actacgccta tgagcaccag gatcgcatca aggccatcgt
4321 gcacatggag agcgtggtgg acgtgatcga gtcctgggac gagtggcctg acatcgagga
4381 ggacatcgcc ctgatcaagt ctgaggaggg cgagaagatg gtgctggaga acaatttctt
4441 tgtggagacc gtgctgccta gcaagatcat gaggaagctg gagccagagg agttcgccgc
4501 ctacctggag ccctttaagg agaagggcga ggtgcggaga cccacactgt cctggccacg
4561 cgagatccca ctggtgaagg gaggcaagcc tgatgtggtg cagatcgtgc ggaactacaa
4621 tgcctatctg agagcctctg acgatctgcc aaagctgttc atcgagagcg accccggctt
4681 cttttccaat gccatcgtgg agggcgccaa gaagttccca aacaccgagt ttgtgaaggt
4741 gaagggcctg cactttctgc aggaggacgc ccccgatgag atgggcaagt acatcaagag
4801 cttcgtggag agagtgctga agaatgagca gtccggagga ggaggctctc tgaagatcga
4861 ctttggcgat tccgccaggg ccgacgatgc aaggcagctg ttcgtgctgg caggagcagc
4921 agaggagggc tttatgacag cagagctggc aggcgtgatc aagaggctgt ggaaggatag
4981 cggcgtgcag gcctgcttca accggtctag agagtaccag ctgaatgaca gcgccgccta
5041 ctatctgaac gacctggata gaatcgccca gcctaactat atcccaaccc agcaggacgt
5101 gctgaggaca cgcgtgaaga ccacaggcat cgtggagacc cacttcacct tcaaggatct
5161 gcacttcaag atgtttgacg tgggaggaca gaggtccgag cgcaagaagt ggattcactg
5221 cttcgagggc gtgaccgcca tcatcttttg tgtggccctg tctgactacg atctggtgct
5281 ggccgaggat gaggagatga atcggatgca cgagtctatg aagctgttcg atagcatctg
5341 taacaataag tggtttaccg acacatccat catcctgttc ctgaacaaga aggacctgtt
5401 tgaggagaag atcaagaaga gccccctgac catctgctac cctgagtatg ccggctccaa
5461 tacatacgag gaggctgccg cctatatcca gtgtcagttt gaggatctga acaagagaaa
5521 ggacaccaag gagatctata cccacttcac atgcgccacc gatacaaaga acgtgcagtt
5581 cgtgtttgat gccgtgacag acgtgatcat caagaacaat ctgaaggact gtggcctgtt
5641 ctgaggtacc gagctcggat ccactagtcc agtgtggtgg aattctgcag atatccagca
5701 cagtggcggc cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg
5761 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
5821 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
5881 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
5941 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
6001 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
6061 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
6121 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
6181 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
6241 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
6301 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
6361 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
6421 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
6481 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
6541 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
6601 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
6661 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
6721 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
6781 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag
6841 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
6901 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
6961 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
7021 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
7081 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
7141 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
7201 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
7261 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
7321 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
7381 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
7441 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
7501 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
7561 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
7621 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
7681 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
7741 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
7801 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
7861 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
7921 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
7981 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
8041 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
8101 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
8161 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
8221 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
8281 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
8341 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
8401 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
8461 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
8521 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
8581 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
8641 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
8701 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
8761 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
8821 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
8881 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
8941 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
9001 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
9061 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
9121 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
9181 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
9241 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
9301 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
9361 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
9421 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
9481 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
9541 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
9601 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
9661 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
9721 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
9781 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
9841 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
9901 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
9961 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
10021 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
10081 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
10141 aaaagtgcca cctgacgtc
//
